Cyp2e1, oxidative stress and mapk signaling pathways in. The mixture was filtered, and the obtained mass was desic. Therefore, the present study was designed to determine the ethanol induced hepatotoxicity in male albino rats and its prevention by simultaneous administration of silymarin. The degree of protection against liver toxicity was determined by measuring the serum. The protective effects of aqueous extracts of the fruit rind of garcinia indica gie on ethanol induced hepatotoxicity and the probable mechanisms involved in this protection were investigated in rats.
We investigated the effects and the possible mechanisms of silymarin on liver injury induced by acute ethanol etoh administration. As a service to our customers we are providing this early version of the manuscript. The extract is filtered using whattman filter paper. The protective effect of fermented curcuma longa l. Diagnostic biomarkers in liver injury by drugs, herbs, and alcohol. Chronic alcohol consumption causes alcoholinduced lipogenesis and promotes hepatic injury by preventing the oxidation of hepatocellular. Asian journal of pharmaceutical and health sciences. Implication of free radical mechanisms in ethanolinduced cellular injury. Role of ethanol in kava hepatotoxicity request pdf.
Prevention of ethanolinduced hepatotoxicity by fermented. Protective effects of sida veronicaefolia against ethanol. Drug induced hepatotoxicity is the most common reason cited for withdrawal of already approved drugs from the market and accounts for more than 50 percent of cases of acute liver failure in the united states. Ethanol markedly elevated levels of serum aspartate aminotransferase ast and alanine aminotransferase alt in mice.
The free radical scavengers known to be endogenous. Kuntze asteraceae leaves aqueous extract prevents ethano l induced liver damage in rats 1ngueguim tsofack florence, 1mbatchou adolphe, 2donfack jean hubert, 1dzeufiet djomeni desire paul, 1gounoue kamkumo raceline, 1djouwoug noussi clarice, 1kamtchouing pierre and 1dimotheophile. Pdf ethanol induced hepatotoxicity in albino rats researchgate. Cyp2e1, oxidative stress and mapk signaling pathways in alcoholinduced hepatotoxicity lili yanga,b, defeng wua, arthur i. Rosmarinic acid ra, a natural polyphenolic compound, shows some biological activities including. We investigated the effects of hericium erinaceus hem on liver injury induced by acute alcohol administration in mice. Original article antioxidant and hepatoprotective effect. The ethanol induced ulcer was carried in rats by administering ethanol as.
Recent fasting was more common than recent alcohol use among those who suffered hepatotoxicity after a dose of 4 to 10 g of acetaminophen per day p. Jun 30, 2014 the protective effect of fermented curcuma longa l. Original article lycium barbarum extract provides effective. B 250 mgkg and 500 mgkg produced p induced liver marker enzymes and total bilirubin, but its 750 mgkg showed remarkable decline in alt, ast, alp and tb levels, compared to the reference. The phytochemical screening was carried on the leaves extracts of melia azedarach revealed the presence of some active ingredients such as alkaloids, tannins, saponins, phenols, glycosides, steroids, terpenoids and flavonoids. Fc was investigated in male c57bl6 mice under ethanolinduced oxidative stress. The hepatoprotective activity of conyza bonariensis ethanol extract was studied against paracetamol induced hepatotoxicity in mice. This is a pdf file of an unedited manuscript that has been accepted for publication.
Ciprofloxacin cardiotoxicity and hepatotoxicity in humans. It was hoped that this model might prove useful in attempts to establish a cyp2e1dependent ethanol hepatotoxicity system, and to evaluate the effect of antioxidants on the ethanol toxicity, in order to establish linkage between cyp2e1, generation of reactive species and ethanol induced hepatotoxicity. Hepatoprotective activity of lophatherum gracile leaves of ethanol extracts against carbon tetrachlorideinduced liver damage in mice 1qingfeng he, 2yanjie li, 1jinfu liu, 1pingping zhang, 1shijie yan, 1xinyi he and 1ailin zhang 1college of food science and biotechnology, tianjin agriculture university, 300384 xiqin, tianjin, china. Toxicology case studies acetaminophen and liver function. Himh0021 attenuates ethanolinduced liver injury and steatosis in. The animals were pretreated once daily with vehicle 2% vv, tween 80 pe, ea, eo, aq extracts of fmb 100 and 200 mgkg silymarin 100 mgkg, standard. Laboratory for molecular pathobiochemistry and clinical research, max planck institute of molecular physiology, dortmund, germany. Acute alcoholinduced liver injury physiology frontiers. Toxicity of ethanol an overview sciencedirect topics. Male spraguedawjey rats were pair fed a nutritionally adequate liquid diet contain ing either ethanol or isocaloric carbohydrate for 46 wk.
Molecular drug evaluation department, national organization for drug control and research nodcar, giza, egypt. Rats in group 2 which treated with ethanol showed depression and nervous signs when compared to the control rats, while there were no clinical signs observed in rats of group 3 intoxicated with ethanol and treated with silymarin, group 4. There is currently no way to reimburse for the absence of liver function. Leaves of melia azedarach was successively extracted with ethanol against carbon.
Readily diffuses across lipid membranes, accounting for its ubiquitous multiorgan effects. Hepatotoxicity in rats was achieved by intraperitoneal administration of ccl 4 1mlkg. College of pharmacy, raichur584103, india 2 kvsr siddhartha college of pharmaceutical sciences, vijayawada 520010, india. In 14 baboons fed ethanol 50% of total energy for 2 to 5 yr with a stand. Free radicals produced by toxicant metabolism initiate what is termed toxicant cascade 12. Hepatoprotective effect of alhagi sparsifolia against alcoholic liver. Low concentration of ethanol induce apoptosis in hepg2 cells. Evaluation of hepatoprotective activity by ethanol induced hepatotoxicity in rats the rats were divided into 5 groups of 6 animals n6 in each kapoor et al. Original article intestinal alkaline phosphatase attenuates alcoholinduced hepatosteatosis in mice sulaiman r.
Liver damage was induced in rats by administering ethanol 5 gkg, 20% wv p. The protective effects of aqueous extracts of the fruit rind of garcinia indica gie on ethanolinduced hepatotoxicity and the probable mechanisms involved in this protection were investigated in rats. From the present study, it can be concluded that ethanolinduced stress can be partly prevented by tocopheryl acetate, and showed best result. Twentyone patients 43% ingested acetaminophen for therapeutic purposes. Ramirez1 aaron karas1 wei liu1 dong hu1 abeba teshager1 sarah shireen gul1 konstantinos p. Because control and silymarin values were virtually identical, only control treatment is shown for ease of viewing. Hepatotoxicity in often due to increase free radical. Silymarin protects against acute ethanolinduced hepatotoxicity in. Rosmarinic acid attenuates the hepatotoxicity induced by. Ethanol consumption is associated with a spectrum of diseases in the liver ranging from steatosis, alcoholic hepatitis to cirrhosis and hep. Fatty liver, also known as afld, is the earliest sign of alcoholinduced liver injury. Phytochemical studies and hepatoprotective activity of. Mice received ethanol 5 gkg bw by gavage every 12 hrs for a total of 3 doses.
Hepatoprotective and antioxidant effects of ethanol. Protective effects of phytoextract blend against ethanolinduced. However, mice receiving fc prior to ethanol treatment did not display hepatotoxicity as. Association of acetaminophen hepatotoxicity with fasting and.
Both hispolon and pm showed a similar peak at the retention time of 6 min. Supplementation of lecithin showed better effect compared to abstination from alcohol on reversing the effect of ethanol induced liver damage in the present study. From the division of digestive and liver diseases, department of internal medicine, university of texas southwestern medical center, dallas. Ethanol etoh causes a further substantial amount of liver insufficiencies world wide. Cyp2e1, oxidative stress and mapk signaling pathways in alcohol induced hepatotoxicity lili yanga,b, defeng wua, arthur i. Moreover, ethanol consumption was considered an issue in kava hepatotoxicity for various cases li and ramzan, 2010, but this point is not any more mentioned, possibly due to lack of relevance.
This implied that pm did contain the active ingredient of hispolon. Original article antioxidant and hepatoprotective effect of. Letter of support and the retraction notification have been preserved as files, kept in the private. Hepatoprotective effect of urine of onehumped camel. Research abstract on ascorbic acid role in the regression of ethanol induced hepatotoxicity in the proceedings of thirtyninth. The effects of this lecithin as a hepatoprotective drug on body weight and antioxidant status of ethanol exposed rats were studied. Research abstract on ascorbic acid role in the regression of ethanol induced hepatotoxicity in. Protective effects of commelina benghalensis linn root extract on ethanol induced acute hepatotoxicity in rats sambrekar 1sudhir n, patil suhas a2 dept of pharmacology, maratha mandals college of pharmacy, belgaum, india, dept of pharmacognosy, maratha mandals college of pharmacy, belgaum, india. Impact of thiamine supplementation in the reversal of ethanol induced toxicity in rats. Effect of lecithin in the treatment of ethanol mediated. Hepatoprotective activity of methanolic extract of.
Screening of liver protective effect of ethanolic leaf extract of callicarpa lanata eecl in ethanol induced hepatotoxicity in wister rats article pdf available february 2019 with 91 reads. Hem 200 mgkg bw was gavage before ethanol administration. Guidelines for manuscript type and related ethics documents. The results of present work indicate that ajme has a significant protective effect against ethanol induced hepatotoxicity in chicks, which may be due to their antioxidant potential. Animals were fed liquid diets and consumed approximately 60 diet per day.
Ethanol cytotoxicity to a transfected hepg2 cell line. Alcohol induces fatty liver by increasing the ratio of reduced form of. This study is one of the very few studies that have investigated the effect ethanol stem bark extract of irvingia gabonensis against sodium arseniteinduced hepatotoxicity in. Hepatoprotective activity of lophatherum gracile leaves of.
Cederbauma, adepartment of pharmacology and systems therapeutics, box 1603, icahn school of medicine at mount sinai, new york, ny, 10029, usa. Low concentration of ethanol induce apoptosis in hepg2. Abstination from alcohol also involved for little hepatic regeneration. Alcohol consumption is customary in most cultures and alcohol abuse is common worldwide. Association of acetaminophen hepatotoxicity with fasting. Hepatoprotective activity of methanolic extract of rhyncosia. Intestinal alkaline phosphatase attenuates alcoholinduced.
The chart showing pdf series, word series, html series, scan qr codes. Protective effects of propolis and probiotic lactobacillus. Alhagi sparsifolia significantly alleviated alcoholinduced liver injury. Materials and methods preparation of the lycium barbarum extract to prepare the lb extract, 50 g of shadedried, ground lb was soaked in 500 ml of ethanol for 7 days at ambient temperature. Ethanol induced hepatotoxicity hepatoprotective study was carried out as described by gujrati and co. From the present study, it can be concluded that ethanol induced stress can be partly prevented by tocopheryl acetate, and showed best result. Rosmarinic acid attenuates the hepatotoxicity induced by ethanol in rats.
Original article alternative therapeutic approaches. B 250 mgkg and 500 mgkg produced p root extract on ethanol induced acute hepatotoxicity in rats sambrekar 1sudhir n, patil suhas a2 dept of pharmacology, maratha mandals college of pharmacy, belgaum, india, dept of pharmacognosy, maratha mandals college of pharmacy, belgaum, india. Effect of lecithin in the treatment of ethanol mediated free. All patients with hepatotoxicity took more than the recommended limit of 4 gd. Its major influence is on the important cause of liver injury.
In the present study, hepatoprotective activity of methanolic extract of rhyncosia beddomei baker leaves in ethanol induced hepatotoxicity was investigated. Rats of groups 2 and 3 received 100mgkg propolis dissolved in a mixture of water and ethanol 1. Hepatoprotective effect of ethanol extract of tubers of momordica tuberosa cogn. Because we had found that ethanol interacts with retinol, we investigated whether it also affects its precursor, carotene. Alcohol is harmful to the body, causing hepatic steatosis, alcoholic hepatitis and cirrhosis. The activity was assessed against ethanol induced hepatotoxicity by measuring the levels of serum enzymes like sgot sgpt and alp, total proteins, total bilirubin, and triglycerides. Pdf screening of liver protective effect of ethanolic. Essential knowledge and tools for working in todays lab, conference presentations, toxicology case studies acetaminophen and liver function. Lee at the division of digestive and liver diseases, university of texas southwestern medical center, 5323 harry hines blvd. Pdf twelve male albino rats of 68 weeks old, weighing 150200 gm each were divided into two groups of 6 rats each. In highperformance liquid chromatography hplc analysis, the. Hamarneh1 byeongmoo kim2 kanakaraju kaliannan1 sara a. Noemi robles, josefa sabria, in reproductive and developmental toxicology, 2011.
Protective effects of sida veronicaefolia against ethanol induced hepatotoxicity in experimental animals ajay sharma, balakrishnan sangameswaran2, suresh chandra mahajan3, manmeet singh saluja1 1department of pharmacy, suresh gyan vihar university, jaipur, rajasthan, india. Ajme 200 mgkg bw and silymarin 50 mgkg bw cotreatment prevented all the changes observed with ethanoltreated rats. The results of the present study of the use of camel urine against alcohol hepatotoxicity in rats indicated a significant hepatoprotective effect. Hepatoprotective activity of ethanol extract of conyza b. Ajme 200 mgkg bw and silymarin 50 mgkg bw cotreatment prevented all the changes observed with ethanol treated rats.
Ethanolinduced free radicals and hepatic dna strand breaks are prevented in vivo by antioxidants. Antiulcerogenic activities of agelanthus dodoneifolius. It was hoped that this model might prove useful in attempts to establish a cyp2e1dependent ethanol hepatotoxicity system, and to evaluate the effect of antioxidants on the ethanol toxicity, in order to establish linkage between cyp2e1, generation of reactive species and ethanolinduced hepatotoxicity. The swiss albino rats 180200g were divided into five groups n6. The results were compared with the effects of tocopheryl acetate. There is a great amount of evidence of the toxicity of ethanol during brain development. Effect of hesperidin and ascorbic acid on liver marker enzymes in serum of ethanol induced hepatotoxic rats 96 5. Protective effect of hericium erinaceus on alcohol induced. Used as an additive in gasoline, as a diluent or solvent, in many household products, and in pharmaceuticals its metabolism. Thirty rats were randomly and equally divided to six groups. The effects of this lecithin as a hepatoprotective drug on body weight and antioxidant status of ethanolexposed rats were studied. Fc was investigated in male c57bl6 mice under ethanol induced oxidative stress. Hepatoprotective effect of urine of onehumped camel camelus.
It is fully miscible in water and is lipid soluble. Furthermore, to understand the mechanisms of its pharmacological actions, the in vivo antioxidant activity and major phyto. A clinical guide to common drugnutrient interactions. Effect of hesperidin and ascorbic acid on serum total protein, albumin and bilirubin in ethanol induced hepatotoxic rats 94 4. Methods nineweekold mice were divided into 4 groups, control, silymarin treatment, etoh treatment, and silymarinetoh treatment, with 6 mice in each group. The study proved that administration of cloves at dose of 500mgkg and cardamom at dose of 500mgkg succeeded in decreasing liver function enzyme levels of alt, ast, alp and ggt.
Autophagy reduces acute ethanolinduced hepatotoxicity and. Ramachandra setty3 1 department of pharmacognosy, v. Autophagy reduces acute ethanol induced hepatotoxicity and steatosis in mice. Increased hepatotoxicity of acetaminophen after chronic. Decarti aliuhvl rtwardi and treatment center, section of liver disease and nutrition, bronx va medical centerand mount sinai school o medicine, new york, ny, united slates of america historical perspective the association between. The protective effects of betaine in ethanol hepatotoxicity were investigated in 24 female wistar albino rats. Ciprofloxacin cardiotoxicity and hepatotoxicity in humans and animals elias adikwu, nelson brambaifa department of pharmacology, faculty of basic medical sciences, college of health sciences, university of port harcourt, choba rivers state, nigeria.
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